KMID : 0613820060160040704
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Journal of Life Science 2006 Volume.16 No. 4 p.704 ~ p.709
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Characterization of Expression of UV-Inducible Gene ( UV100 and UV150) in Caenorhabditis elegans
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Shin Sue-Hwa
Choi Eun-Young Choi In-Soon
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Abstract
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The present study intends to characterize the DNA damage-inducible responses in Caenorhabditis elegans. To study UV-inducible responses in C. elegans, two UV-inducible cDNA clones were isolated from C. elegans by using subtration hybridization method. To investigate the expression of isolated genes, UV100 and UV150, the cellular levels of the transcript were determined by Northern blot analysis after UV-irradiation. The transcripts of isolated gene increased rapidly and reached maximum accumulation after UV-irradiation. Compared to the message levels of control, the levels of maximal increase were approximately 2 folds to UV-irradiation. These results implied that the effects of damaging agents are complex and different regulatory pathways exist for the induction of these genes. To study the function of UV100 and UV150 gene in response to UV irradiation, we carried out a RNAi experiment and investigated the UV sensivity. This result indicated that UV100 gene involved in stage-specific repair pathway or regulated by development.
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KEYWORD
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C. elegans, UV100 and UV150 UV-inducible gene, RNAi, DNA Damage, DNA repair, cell viability
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